The Magic of Cake Mix

When I was first learning to bake, I always wondered what was so special about ready-made mixes. In my mind, cake really wasn’t that complicated. Most cake recipes that I found online always called for some combination of flour, sugar, eggs, salt, and butter. So, why was it that the boxed cake mix always resulted in the same, consistent success, while baking from scratch yielded uncertain results?

What is in Cake Mix?

Cake mix is a box of conveniently measured out dry ingredients. All the baker needs to do is add eggs, some kind of fat, like oil, and a liquid, like water or milk. But what exactly makes up those dry ingredients?

According to the Betty Crocker website, their yellow cake mix consists of quite recognizable ingredients, including flour, sugar, corn syrup, baking soda, and corn starch. These match the ingredients one could think of in a normal recipe. The flour makes up most of the cake, while the sugar and corn syrup are for sweetness. The baker would add in the water, eggs, and fat (vegetable oil) on their own, so the question lies in the rest of the ingredients. 

The Extra Additives

The rest of the ingredients of the Betty Crocker yellow cake mix make up less than 2% of the ingredients. However, they are extremely important to the stability of the cake. According to the label, these ingredients are monoglycerides, dicalcium phosphate, sodium stearoyl lactylate, xanthan gum, and cellulose gum. These ingredients are additives that help maintain the texture, stability, and shelf life of the mix. 

    • For instance, dicalcium phosphate is a tasteless, odorless additive that is usually included along with flour. It is meant to break up the ingredients evenly in the baking process and improve the consistency of the flour (Enax et al., 2022).
    • Additionally, sodium stearoyl lactate is an additive that is also used as a stabilizer and emulsifier in processed foods (FAO & WHO, 1996). As a stabilizer, its job is to make sure that the food’s texture and taste maintain their quality during extended times on the shelf. As an emulsifier, its job is to allow ingredients like oil and water to mix together.
    • Xanthan gum is also an ingredient commonly used in both food, polishes, and cleaners. Its job is to act as a stabilizer and thickener in convenience foods. More specifically, its job is to retain moisture in bakery goods and trapping gas in ready-made cake mixes so that they bake better (Katzbauer, 1997). 

These are just a few of the extra ingredients that make cake mixes as consistent and convenient as they are today. They hold the ingredients together, enhance the taste and texture of the cake, and ensure that the results are similar each time. So, while, yes, cake mix is essentially the same mixture of flour and sugar than one might expect to find in a regular cake, there is a bit of science involved to help make sure it’s a bit easier each time!

 

References

Betty Crocker (n.d.). Betty Crocker Favorites Super Moist Yellow Cake Mix, Holiday Baking, 13.25 oz https://www.bettycrocker.com/products/betty-crocker-baking-and-cake-mixes/yellow 

Enax, J., Meyer, F., Schulze Zur Wiesche, E., & Epple, M. (2022). On the Application of Calcium Phosphate Micro- and Nanoparticles as Food Additive. Nanomaterials (Basel, Switzerland), 12(22), 4075. https://doi.org/10.3390/nano12224075 

FAO & WHO. (1996). SODIUM STEAROYL-2-LACTYLATE. Joint FAO/WHO Expert Committee on Food Additives (JECFA) Monographs. https://www.fao.org/fileadmin/user_upload/jecfa_additives/docs/Monograph1/Additive-424.pdf 

Katzbauer, B. (1997). Properties and applications of xanthan gum. Polymer Degradation and Stability, Vol, 59, Iss. 1–3, https://doi.org/10.1016/S0141-3910(97)00180-8 

Maehasi, N (2020). My very best Vanilla Cake – stays moist 4 days! https://www.recipetineats.com/my-very-best-vanilla-cake/ 

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  Timothy Durham was convicted of rape when a test showed his DNA genotype matched the DNA recovered from the crime scene. Eye witnesses testified that Durham was in a different state at the time of the incident, but he wasn’t exonerated from his 3000 year sentence until follow up DNA analysis showed that the preliminary forensic analysis used in the trial was misinterpreted (Thomas et al.). DNA testing serves an invaluable part in our criminal justice system, but it comes with complications and bias that can lead to wrongful convictions and misinformed juries.  

Background:

Forensic genetics aims to identify the origin of a biological sample. It can be traced back to Karl Landsteiner who discovered human ABO blood groups. With blood groups, suspects could be eliminated based on their blood type. For example, if a suspect had type A blood and there was type B blood left at the scene by the perpetrator, the investigators could rule out the suspect. Unfortunately, the reverse didn’t work. If the suspect and perpetrator both had the same blood type, it can’t be concluded that the suspect was guilty. Blood identifiers evolved into 8 distinct categories which improved the ability to eliminate suspects, but could only conclude that (if the system matched) it might be the perpetrator or this person might be related to the perpetrator (Jobling and Gill). The field evolved in the 1980s with the discovery of Minisatellites, lines of repetitive DNA that don’t encode proteins. Minisatellite patterns in DNA create what we know today as the DNA fingerprint, and it has a much higher accuracy rate than previous methods (Augustyn et al.). Around the same time, a breakthrough for victims of sexual assault was discovered. In the case of assault, bodily fluids of both the victim and the assaulter can get mixed. A process called differential lysis was created which could selectively increase the concentration of sperm in a fluid sample thereby allowing for accurate DNA testing (Jobling and Gill). Forensics evolved to use single-locus probe testing after several years because of its ease of interpretation. It looked at specific minisatellites that had high variability between subjects. This was the first DNA-based method to be used in a criminal investigation. Two murder homicides from several years apart were connected using DNA evidence. The evidence was then used to exonerate an innocent man, and the guilty party was found when he tried to evade mandatory DNA testing for all men in the small town. His guilt was then reinforced when the DNA profiles matched, and he gave a confession (Jobling and Gill). Finding DNA at a crime scene doesn’t guarantee that you’ll find a murderer. There are issues with non-pure samples and limited samples that make DNA work difficult, and accreditation of facilities and new techniques and take time (Jobling and Gill).  

Subjectivity of DNA Interpretation:

In a 2009 publication, the National Academy of Science discusses the possibility for subjective influence in lab based forensics. The interpretation bias happens in complex cases where, for example, there could be a mixture of DNA. A study published in 2011 looked specifically at this issue. A gang rape happened in Georgia, and the mixture in DNA from the case was used to prosecute one suspect after another suspect took a plea bargain to testify against him. With DNA mixtures, there are three possible conclusions: suspect excluded, suspect cannot be excluded, and inconclusive. If the suspect is excluded, it means that there’s little to no possibility that his DNA is part of the sample. If the suspect cannot be excluded, it means that there’s a possibility that his DNA is part of the sample. In the trial, the DNA examiners who knew the context of the case concluded that the suspect being prosecuted could not be excluded. The 2011 study gave the DNA evidence from this case and the DNA profiles of the suspects to 17 forensic examiners. These examiners had no context of the case, and they all worked independently. Of the 17, only 1 came to the same conclusion (suspect cannot be excluded) as the examiners who knew the context of the case. Twelve of the 17 concluded that the suspect who was prosecuted and convicted had no relation to the DNA found in the sample. Not only does this show that subjectivity occurs due to the context of the case, but the 17 examiners came up with different findings based on their past experiences meaning that not all DNA analysis is objective. Some organizations like the Scientific Working Group on DNA Analysis Methods say that quantitative statistical evidence should be presented with every categorical conclusion, but the US doesn’t require this (Dror and Hampikian).

False Positives:

There are two categories that cause wrongful identification in a DNA report: coincidental matches and false positives. Coincidental matches are DNA matches between two unrelated people. The probability of these matches is about 1 to 500,000 for white individuals and about 1 to 350,000 for black individuals. This small probability of coincidental matches makes people feel confident in DNA evidence. False positives, on the other hand, are matches between DNA and test DNA that result from issues in the forensics lab. There have been multiple cases of forensics labs accidentally switching victim and suspected rapist DNA which lead to convictions that were later overturned when the lab realized the error. There are also cases of misreading or misunderstanding a sample. In a case from 1993, a man was convicted of rape because someone interpreted a mixed sample of victim and perpetrator DNA as an individual sample. The conviction was again overturned. To prevent false convictions, retesting DNA is allowed, but it’s not an efficient solution. Many people are pushing for statistical information on the probability of false positives, so that the jurors can make accurate judgements based on DNA evidence (Thomas et al.).  

Conclusion:

DNA analysis is a useful tool for the criminal justice system, but subjectivity can be an issue in forensic testing. To combat wrongful convictions, there are organizations like The Innocence Project who use DNA testing to free the innocent, but reformed forensic testing standards should be put in place to prevent subjectivity and to ensure juries are correctly informed during trials. https://www.innocenceproject.org/    

Citation:

Augustyn , Adam, et al. “DNA Finerprinting.” Encyclopedia Britannica , 26 Apr. 2018, www.britannica.com/science/DNA-fingerprinting#ref153186. Dror, Itiel E., and Greg Hampikian. “Subjectivity and Bias in Forensic DNA Mixture Interpretation.” Science & Justice, vol. 51, no. 4, 2011, pp. 204–208., doi:10.1016/j.scijus.2011.08.004. Jobling, Mark A., and Peter Gill. “Encoded Evidence: DNA in Forensic Analysis.” Nature Reviews: Genetics, vol. 5, no. 10, Oct. 2004, pp. 739–751., doi:10.1038/nrg1455. Thompson, William C, et al. “How the Probability of a False Positive Affects the Value of DNA Evidence.” Journal of Forensic Sciences, vol. 48, no. 1, Jan. 2003, pp. 1–8., doi:10.1520/jfs2001171.