Coloring Space Green

“It appears a colonization of the moon could become a reality,” state Cornell University researchers R. W. Langhans and D. R. Dreesen hopefully in 1988 – and it would only be a “true colony [if] food could be produced” (Langhans and Dreesen, 1988).

The quest for interplanetary travel and colonization is one that, in the modern day, remains far-fetched and distant. While governments and research organizations have landed robots on Mars and mapped much of the Moon, the dream of a permanent, or at least self-sustaining, Moon base is still far off in the distance. One of the largest barriers to that dream is the challenge of growing food in space.

Plants grown in space through NASA's Veggie system.

Image Credit: NASA/ISS

“The opportunities are exciting and almost unlimited in scope” (Langhans and Dreesen, 1988)

 

Plant growth in space is challenging for a number of reasons due not only to the harsh conditions of space, but also the harsh design and engineering conditions of the spacecraft. Plants in space need to be able to survive radiation, microgravity, and low-light conditions, while also being physically compact, non-disruptive to the spacecraft’s oxygen and carbon-dioxide levels, and unable to produce excess waste, toxins, and dangerous microbes (Musgrave, 2008; Langhans and Dreesen, 1988; Reed and Vanden Bosch, 2023). Beyond those requirements, these compact space-grown plants must provide all the essential nutrients required for a human diet in the absence of animal products and larger plant products like large fruits (Langhans and Dreesen, 1988).

While difficult, growing plants in space is extremely rewarding. NASA has determined that only eight species of plants – wheat, rice, white potato, sweet potato, soy bean, peanut, lettuce, and sugar beet – contain all the essential elements, carbohydrates, oils, and amino acids for a balanced human diet (Langhans and Dreesen, 1988). Vitamins, while not fully covered by these eight species of plants, can be supplemented for shorter missions, although they break down when prepackaged for longer ones (Langhans and Dreesen, 1988; and NASA, 2023). In addition, plants have been shown to be positively correlated with psychological well-being on Earth and in space (NASA, 2023). Plants then, are not only beneficial for the astronauts physical health, but their mental health too.

Currently, NASA employs two growth habitats: the Vegetable Production System (nicknamed Veggie) and the Advanced Plant Habitat (APH) (NASA, 2023). Both Veggie and the APH use enclosed microbiomes with simulated radiance from LED lights and carefully controlled gas content, temperature, and moisture levels (NASA, 2023). While not all eight of the ideal plants identified in 1988 have been successfully grown, the advancements of Veggie, APH and other new technologies shine a light on a hopeful future of plant-filled space exploration.

 

Credits

Langhans, R. W., & Dreesen, D. R. (1988). Challenges to plant growing in space. HortScience, 23(2), 286-293.

Musgrave, M. E. (2008). Growing plants in space. CABI Reviews. https://doi.org/10.1079/pavsnnr20072065

NASA. (2023, December 8). Growing plants in space. NASA. https://www.nasa.gov/exploration-research-and-technology/growing-plants-in-space/

Reed, D. W., & Vanden Bosch, C. A. (2023). Engineering perspectives of growing plants in space. Journal of the Indian Institute of Science, 103(3), 797–805. https://doi.org/10.1007/s41745-023-00369-6

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  Timothy Durham was convicted of rape when a test showed his DNA genotype matched the DNA recovered from the crime scene. Eye witnesses testified that Durham was in a different state at the time of the incident, but he wasn’t exonerated from his 3000 year sentence until follow up DNA analysis showed that the preliminary forensic analysis used in the trial was misinterpreted (Thomas et al.). DNA testing serves an invaluable part in our criminal justice system, but it comes with complications and bias that can lead to wrongful convictions and misinformed juries.  

Background:

Forensic genetics aims to identify the origin of a biological sample. It can be traced back to Karl Landsteiner who discovered human ABO blood groups. With blood groups, suspects could be eliminated based on their blood type. For example, if a suspect had type A blood and there was type B blood left at the scene by the perpetrator, the investigators could rule out the suspect. Unfortunately, the reverse didn’t work. If the suspect and perpetrator both had the same blood type, it can’t be concluded that the suspect was guilty. Blood identifiers evolved into 8 distinct categories which improved the ability to eliminate suspects, but could only conclude that (if the system matched) it might be the perpetrator or this person might be related to the perpetrator (Jobling and Gill). The field evolved in the 1980s with the discovery of Minisatellites, lines of repetitive DNA that don’t encode proteins. Minisatellite patterns in DNA create what we know today as the DNA fingerprint, and it has a much higher accuracy rate than previous methods (Augustyn et al.). Around the same time, a breakthrough for victims of sexual assault was discovered. In the case of assault, bodily fluids of both the victim and the assaulter can get mixed. A process called differential lysis was created which could selectively increase the concentration of sperm in a fluid sample thereby allowing for accurate DNA testing (Jobling and Gill). Forensics evolved to use single-locus probe testing after several years because of its ease of interpretation. It looked at specific minisatellites that had high variability between subjects. This was the first DNA-based method to be used in a criminal investigation. Two murder homicides from several years apart were connected using DNA evidence. The evidence was then used to exonerate an innocent man, and the guilty party was found when he tried to evade mandatory DNA testing for all men in the small town. His guilt was then reinforced when the DNA profiles matched, and he gave a confession (Jobling and Gill). Finding DNA at a crime scene doesn’t guarantee that you’ll find a murderer. There are issues with non-pure samples and limited samples that make DNA work difficult, and accreditation of facilities and new techniques and take time (Jobling and Gill).  

Subjectivity of DNA Interpretation:

In a 2009 publication, the National Academy of Science discusses the possibility for subjective influence in lab based forensics. The interpretation bias happens in complex cases where, for example, there could be a mixture of DNA. A study published in 2011 looked specifically at this issue. A gang rape happened in Georgia, and the mixture in DNA from the case was used to prosecute one suspect after another suspect took a plea bargain to testify against him. With DNA mixtures, there are three possible conclusions: suspect excluded, suspect cannot be excluded, and inconclusive. If the suspect is excluded, it means that there’s little to no possibility that his DNA is part of the sample. If the suspect cannot be excluded, it means that there’s a possibility that his DNA is part of the sample. In the trial, the DNA examiners who knew the context of the case concluded that the suspect being prosecuted could not be excluded. The 2011 study gave the DNA evidence from this case and the DNA profiles of the suspects to 17 forensic examiners. These examiners had no context of the case, and they all worked independently. Of the 17, only 1 came to the same conclusion (suspect cannot be excluded) as the examiners who knew the context of the case. Twelve of the 17 concluded that the suspect who was prosecuted and convicted had no relation to the DNA found in the sample. Not only does this show that subjectivity occurs due to the context of the case, but the 17 examiners came up with different findings based on their past experiences meaning that not all DNA analysis is objective. Some organizations like the Scientific Working Group on DNA Analysis Methods say that quantitative statistical evidence should be presented with every categorical conclusion, but the US doesn’t require this (Dror and Hampikian).

False Positives:

There are two categories that cause wrongful identification in a DNA report: coincidental matches and false positives. Coincidental matches are DNA matches between two unrelated people. The probability of these matches is about 1 to 500,000 for white individuals and about 1 to 350,000 for black individuals. This small probability of coincidental matches makes people feel confident in DNA evidence. False positives, on the other hand, are matches between DNA and test DNA that result from issues in the forensics lab. There have been multiple cases of forensics labs accidentally switching victim and suspected rapist DNA which lead to convictions that were later overturned when the lab realized the error. There are also cases of misreading or misunderstanding a sample. In a case from 1993, a man was convicted of rape because someone interpreted a mixed sample of victim and perpetrator DNA as an individual sample. The conviction was again overturned. To prevent false convictions, retesting DNA is allowed, but it’s not an efficient solution. Many people are pushing for statistical information on the probability of false positives, so that the jurors can make accurate judgements based on DNA evidence (Thomas et al.).  

Conclusion:

DNA analysis is a useful tool for the criminal justice system, but subjectivity can be an issue in forensic testing. To combat wrongful convictions, there are organizations like The Innocence Project who use DNA testing to free the innocent, but reformed forensic testing standards should be put in place to prevent subjectivity and to ensure juries are correctly informed during trials. https://www.innocenceproject.org/    

Citation:

Augustyn , Adam, et al. “DNA Finerprinting.” Encyclopedia Britannica , 26 Apr. 2018, www.britannica.com/science/DNA-fingerprinting#ref153186. Dror, Itiel E., and Greg Hampikian. “Subjectivity and Bias in Forensic DNA Mixture Interpretation.” Science & Justice, vol. 51, no. 4, 2011, pp. 204–208., doi:10.1016/j.scijus.2011.08.004. Jobling, Mark A., and Peter Gill. “Encoded Evidence: DNA in Forensic Analysis.” Nature Reviews: Genetics, vol. 5, no. 10, Oct. 2004, pp. 739–751., doi:10.1038/nrg1455. Thompson, William C, et al. “How the Probability of a False Positive Affects the Value of DNA Evidence.” Journal of Forensic Sciences, vol. 48, no. 1, Jan. 2003, pp. 1–8., doi:10.1520/jfs2001171.