Hey Kids: Brush your teeth, stay in school… and read the citations!

A co-worker sent this out to our research group in a rare moment of light-heartedness in the otherwise all-work-no-play world of physical chemistry research. His take was that we, as disseminators of scientific research, must be careful not to mislead the impressionable public. My take, which is admittedly more jaded, was that once again non-science media cannot be trusted to re-interpret the work of specialists. Of course a certain amount of external scrutinizing is required to keep we the bespectacled on the straight and narrow, otherwise we’d be spending all our grant money on Ferraris and replica iron man suits, but I’d like to imagine there is a happy medium between the far-fetched interpretations of science and technology that leave people feeling short changed  and some legitimately useful marriage of cutting edge scientific research and mass media.

While trolling the interwebs for humorous tie-ins to this article, I came across a few pieces on the topic of science journalism written by Ananyo Bhattacharya, the online editor for Nature magazine, who in his own words has turned to the “dark side” of journalism after getting a PhD in physics and working as a researcher. Dark side is right, considering his views on the role of journalists covering science is basically that they have zero obligation to accurately portray scientific research as long as no explicit lies are told. I was particularly dumbfounded on his take regarding a concept of “copy-checking”.  Considering I am not a famous enough scientist to have articles written about my research I had never heard of copy-checking, but the basic idea is that journalists should not allow the scientists who they are writing about to preview their articles before submission. Ostensibly, this is to prevent scientists from doctoring the article to shed a more favorable light on their work, which I guess is an institutional convention for journalists to prevent politicians or other public figures from massaging press releases. That’s all well and good, but considering scientific researchers are supposed to be following rigorous ethical guidelines anyways, especially regarding publications and authorship, this seems to be a superfluous condition. Nature publishing group (who employs the journalist mentioned above) explicitly states the following regarding the requirements of authors.

“Submission to a Nature journal is taken by the journal to mean that all the listed authors have agreed all of the contents.”

That is to say, if your name appears on the work you are tacitly vouching for its content. Why the same consideration should not be extended to “non-scientific” reporting of research results I don’t know, but I digress. There is a simple solution to the ancient struggle between science and the media: read the references yourself. If you ever find yourself in the strange land of pseudo-scientific explanations or questionable presentation of the latest research, quit while you’re ahead and go straight to the source of the research being reported. Presumably even bad science journalism should cite the source of their topics, maybe even including the title of a research publication. A quick search in your favorite internet search engine will usually take you straight to the desired publication, and if you’re on a college campus you can probably even access the entire article gratis. Even reading the abstract (a brief overview of the research methods and findings used in the article) can go a long way in telling you with how much vigor you should roll your eyes.

But what possible consequence could bad reporting of scientific research have? In some of the most esoteric research areas I’d say very little. For example, when the research occurring at the CERN Large Hadron Collider is misrepresented or even completely wrong in a news report, likely only the most familiar with their research (that excludes me!) would ever notice. Meanwhile, a piece on climate change with an appropriately alluring title might encourage residents of southern Florida to head for the hills. Medical research may be the one exception in which media claims are taken with a grain of salt, maybe because they’ve got this guy who makes this article feel a little redundant, but I’ll just tell myself we’re on the same team.

Bottom line: you owe it to yourself as an inquisitive person (you ARE reading Charged Magazine after all) to apply a healthy dose of skepticism to any scientific report or study. The whole point of science after all is not to persuade with vagaries, but to convince with facts.

Sources:

http://www.nature.com/authors/policies/authorship.html

http://www.memegenerator.net

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  Timothy Durham was convicted of rape when a test showed his DNA genotype matched the DNA recovered from the crime scene. Eye witnesses testified that Durham was in a different state at the time of the incident, but he wasn’t exonerated from his 3000 year sentence until follow up DNA analysis showed that the preliminary forensic analysis used in the trial was misinterpreted (Thomas et al.). DNA testing serves an invaluable part in our criminal justice system, but it comes with complications and bias that can lead to wrongful convictions and misinformed juries.  

Background:

Forensic genetics aims to identify the origin of a biological sample. It can be traced back to Karl Landsteiner who discovered human ABO blood groups. With blood groups, suspects could be eliminated based on their blood type. For example, if a suspect had type A blood and there was type B blood left at the scene by the perpetrator, the investigators could rule out the suspect. Unfortunately, the reverse didn’t work. If the suspect and perpetrator both had the same blood type, it can’t be concluded that the suspect was guilty. Blood identifiers evolved into 8 distinct categories which improved the ability to eliminate suspects, but could only conclude that (if the system matched) it might be the perpetrator or this person might be related to the perpetrator (Jobling and Gill). The field evolved in the 1980s with the discovery of Minisatellites, lines of repetitive DNA that don’t encode proteins. Minisatellite patterns in DNA create what we know today as the DNA fingerprint, and it has a much higher accuracy rate than previous methods (Augustyn et al.). Around the same time, a breakthrough for victims of sexual assault was discovered. In the case of assault, bodily fluids of both the victim and the assaulter can get mixed. A process called differential lysis was created which could selectively increase the concentration of sperm in a fluid sample thereby allowing for accurate DNA testing (Jobling and Gill). Forensics evolved to use single-locus probe testing after several years because of its ease of interpretation. It looked at specific minisatellites that had high variability between subjects. This was the first DNA-based method to be used in a criminal investigation. Two murder homicides from several years apart were connected using DNA evidence. The evidence was then used to exonerate an innocent man, and the guilty party was found when he tried to evade mandatory DNA testing for all men in the small town. His guilt was then reinforced when the DNA profiles matched, and he gave a confession (Jobling and Gill). Finding DNA at a crime scene doesn’t guarantee that you’ll find a murderer. There are issues with non-pure samples and limited samples that make DNA work difficult, and accreditation of facilities and new techniques and take time (Jobling and Gill).  

Subjectivity of DNA Interpretation:

In a 2009 publication, the National Academy of Science discusses the possibility for subjective influence in lab based forensics. The interpretation bias happens in complex cases where, for example, there could be a mixture of DNA. A study published in 2011 looked specifically at this issue. A gang rape happened in Georgia, and the mixture in DNA from the case was used to prosecute one suspect after another suspect took a plea bargain to testify against him. With DNA mixtures, there are three possible conclusions: suspect excluded, suspect cannot be excluded, and inconclusive. If the suspect is excluded, it means that there’s little to no possibility that his DNA is part of the sample. If the suspect cannot be excluded, it means that there’s a possibility that his DNA is part of the sample. In the trial, the DNA examiners who knew the context of the case concluded that the suspect being prosecuted could not be excluded. The 2011 study gave the DNA evidence from this case and the DNA profiles of the suspects to 17 forensic examiners. These examiners had no context of the case, and they all worked independently. Of the 17, only 1 came to the same conclusion (suspect cannot be excluded) as the examiners who knew the context of the case. Twelve of the 17 concluded that the suspect who was prosecuted and convicted had no relation to the DNA found in the sample. Not only does this show that subjectivity occurs due to the context of the case, but the 17 examiners came up with different findings based on their past experiences meaning that not all DNA analysis is objective. Some organizations like the Scientific Working Group on DNA Analysis Methods say that quantitative statistical evidence should be presented with every categorical conclusion, but the US doesn’t require this (Dror and Hampikian).

False Positives:

There are two categories that cause wrongful identification in a DNA report: coincidental matches and false positives. Coincidental matches are DNA matches between two unrelated people. The probability of these matches is about 1 to 500,000 for white individuals and about 1 to 350,000 for black individuals. This small probability of coincidental matches makes people feel confident in DNA evidence. False positives, on the other hand, are matches between DNA and test DNA that result from issues in the forensics lab. There have been multiple cases of forensics labs accidentally switching victim and suspected rapist DNA which lead to convictions that were later overturned when the lab realized the error. There are also cases of misreading or misunderstanding a sample. In a case from 1993, a man was convicted of rape because someone interpreted a mixed sample of victim and perpetrator DNA as an individual sample. The conviction was again overturned. To prevent false convictions, retesting DNA is allowed, but it’s not an efficient solution. Many people are pushing for statistical information on the probability of false positives, so that the jurors can make accurate judgements based on DNA evidence (Thomas et al.).  

Conclusion:

DNA analysis is a useful tool for the criminal justice system, but subjectivity can be an issue in forensic testing. To combat wrongful convictions, there are organizations like The Innocence Project who use DNA testing to free the innocent, but reformed forensic testing standards should be put in place to prevent subjectivity and to ensure juries are correctly informed during trials. https://www.innocenceproject.org/    

Citation:

Augustyn , Adam, et al. “DNA Finerprinting.” Encyclopedia Britannica , 26 Apr. 2018, www.britannica.com/science/DNA-fingerprinting#ref153186. Dror, Itiel E., and Greg Hampikian. “Subjectivity and Bias in Forensic DNA Mixture Interpretation.” Science & Justice, vol. 51, no. 4, 2011, pp. 204–208., doi:10.1016/j.scijus.2011.08.004. Jobling, Mark A., and Peter Gill. “Encoded Evidence: DNA in Forensic Analysis.” Nature Reviews: Genetics, vol. 5, no. 10, Oct. 2004, pp. 739–751., doi:10.1038/nrg1455. Thompson, William C, et al. “How the Probability of a False Positive Affects the Value of DNA Evidence.” Journal of Forensic Sciences, vol. 48, no. 1, Jan. 2003, pp. 1–8., doi:10.1520/jfs2001171.